FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37453434
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1421550.37955131163673805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC175300.088.835469
GGCACTT107400.085.699119
GGCAATG108300.084.54849
TTCAGCG121050.082.626699
TGAACTC121800.082.546839
GGACCTC84550.082.3468869
TACACTT97850.082.123299
GTCAGCG91850.081.541629
TGCACTT115800.081.5332269
GTAACTC98400.081.422949
ACCGCTG92150.081.32779
AGATTGG154600.081.1615149
GGCTGAG198750.081.006419
TCCAGCG72900.080.978629
ACCAGCG49000.080.737589
TGCAGCG93900.080.6212549
AGAACTC96500.080.614549
TTAGGCT113800.080.378679
GTACCTC88850.079.751389
TCCGCTG118300.079.529189
Produced by FastQC (version 0.11.5)