FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41137956
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1568960.38138987751360326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC151500.086.675599
GGCACTT102900.085.4299
GGACCTC108050.085.093219
GGCAATG141650.084.890959
GTACCTC106100.084.73249
GCACGTA91300.082.706699
AGATTGG207700.082.635369
TTCAGCG130650.082.151099
TGATTGG238600.082.124569
GTCAGCG96300.081.5686959
TGAACTC105900.081.035729
TGCAGCG106550.080.942519
TCCAGCG79450.080.93559
GTCCAGT113300.080.772989
ATCACTT90250.080.72219
GGCTGAG200300.080.54329
AACAGCG47350.080.238789
ACACGTA62550.080.101289
AGAACTC79350.079.900349
TGACCTC90950.079.839749
Produced by FastQC (version 0.11.5)