FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39861456
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1415050.354992050465994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC127250.085.841649
GGCACTT89850.084.36129
GGCAATG118050.083.801399
GGACCTC91550.083.4172069
TTCAGCG140700.082.73279
GTCAGCG109450.082.141539
GTACCTC96600.081.3179249
CACAGCG64850.081.071019
TCCAGCG83600.081.067299
AGCAGCG81150.081.056759
TGCAGCG116450.080.91529
AACAGCG49800.080.871319
GGCAGCG91750.080.80259
TGAACTC92700.080.281829
TGACCTC81500.080.242469
ACCAGCG52350.080.0165569
TTAGGCT124800.079.763579
GTAGGCT126200.079.367969
GGCTGAG185350.078.945449
GTCCAGT99950.078.925049
Produced by FastQC (version 0.11.5)