FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40906548
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1373510.3357677602128637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC168150.087.781229
GGCACTT139750.086.181669
GGACCTC112500.085.8649449
GTACCTC110200.084.898919
GGCAATG109300.083.55589
TGCACTT142550.082.689829
TACACTT130450.082.2048349
ATCACTT119800.082.05999
TGAACTC118750.081.705679
TGACCTC91300.081.614939
GTCCAGT141600.081.3999259
GTAACTC100150.081.183979
AGAACTC91850.081.022819
TAACCTC74650.079.651149
ACCGCTG77450.079.224339
GTCAGCG70500.079.220159
CACACTT83700.079.1527949
TGCAGCG75750.079.058759
GGACGCT138800.078.867669
GTCACTT93050.078.599879
Produced by FastQC (version 0.11.5)