FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38688246
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1342370.3469710154345069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC176700.089.150479
GGCACTT111350.086.026139
TGAACTC124200.084.5438169
GGCAATG110200.084.4674159
GGACCTC112600.084.3541269
GTACCTC113650.084.201599
AGATTGG192600.084.1825269
TTCAGCG134550.084.005559
TGATTGG214800.082.999359
GTCAGCG105950.082.431949
CACAGCG63550.082.278529
GGCTGAG201200.082.212959
AACAGCG50850.082.094239
GCATAGC88000.082.030559
TGCACTT116550.081.94359
ATATAGC85050.081.86059
TGCAGCG100400.081.832749
GTATAGC109450.081.488179
TAATAGC88200.081.467639
GTAACTC107750.081.36349
Produced by FastQC (version 0.11.5)