FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43297086
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1406200.3247793627497241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC158100.087.9242869
GGCACTT132500.086.954689
GTACCTC135400.084.320639
GGACCTC137750.083.744059
GGCAATG118900.083.7196669
TGCACTT139750.082.6475149
AGATTGG181000.082.441469
GCACCTC78450.082.270589
ACCGCTG114450.081.829369
TGACCTC110800.081.78189
ATCACTT120700.081.684219
AACGCTG112850.081.4745259
CTCGCTG153050.081.2060559
TACACTT133400.080.849889
TGAACTC119250.080.845479
GTCAGCG93950.080.829479
AGAACTC89250.080.669449
GTAACTC98850.080.522169
TGATTGG204850.080.447329
TCCGCTG166500.080.1512159
Produced by FastQC (version 0.11.5)