FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40182258
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1355910.33743997164121536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC192200.088.463329
GGCACTT101800.086.682529
GGACCTC111000.084.76089
GTACCTC121150.083.735849
GGCAATG113300.083.291669
GCACGTA84750.082.7142269
GTAACTC111650.082.693459
AGAACTC99100.082.5264369
AGATTGG173700.082.4641349
TGAACTC132750.082.0357369
TGCAGCG96550.081.9513859
TTCAGCG122500.081.4560859
GGCTAAG146200.081.310589
GTCAGCG88550.081.095519
TTAGGCT139850.080.995239
TCCAGCG70200.080.576479
TGACCTC91850.080.301919
TGATTGG204900.079.874279
TGCACTT117150.079.662489
GGCTGAG180550.079.361779
Produced by FastQC (version 0.11.5)