FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37366445
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1170660.31329177822508936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC158550.087.4653859
GGCACTT110000.086.262569
GTCAGCG92550.084.053749
TTCAGCG135350.083.7906659
TTAGGCT132600.083.2729
GGCAATG105900.083.0996259
GGCTAAG157150.083.016399
AACAGCG51750.082.594619
GGACCTC83450.082.292749
AGAACTC86150.081.918719
GTACCTC89350.081.74889
ATCACTT92450.081.47349
AGATTGG159700.081.4529349
GGCAGCG65450.081.3421259
TCCAGCG81950.081.2491159
TGCAGCG101300.081.153499
TGAACTC109000.080.9105159
ACCGCTG81150.080.821099
TGCACTT116150.080.631979
AGCAGCG73200.080.580529
Produced by FastQC (version 0.11.5)