FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45794985
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1335910.29171534830724366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC160550.087.885559
GGACCTC124600.084.922429
GGCACTT106350.084.669319
GTCAGCG106550.083.262349
GGCAATG133050.082.9200449
AACAGCG52050.082.667099
TGAACTC123650.082.65589
GTACCTC120850.082.5666359
TGACCTC113450.081.924079
ACCGCTG103500.081.6779569
ACACGTA55450.081.623759
TGCAGCG103300.081.4682859
GTCCAGT118600.081.4500669
TTAGGCT153500.081.0312049
AGAACTC92750.080.750089
TCCGCTG167300.080.6776669
AGATTGG162850.080.520019
GTAACTC109500.080.455359
ATACCTC86450.080.426899
TAATAGC100350.080.171729
Produced by FastQC (version 0.11.5)