FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n02_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n02_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41241391
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1438810.3488752355612835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTC167750.086.888479
GGCACTT130350.086.022649
GTCAGCG111550.083.703149
GGCAATG113550.083.6090859
TGAACTC122700.082.87059
TTCAGCG157250.082.572559
TACACTT120050.082.405269
AACAGCG52050.081.66399
GTAACTC97200.081.6465769
AGCAGCG88500.081.193929
TGCAGCG127100.081.169
ATCACTT118450.080.992389
AGAACTC92900.080.926929
GGCAGCG93900.080.823759
GTCCAGT120100.080.749639
CACAGCG66300.080.658979
TGCACTT142800.080.485019
GTCACTT87450.080.2680059
GGACCTC76700.080.1247569
AGATTGG160200.080.044059
Produced by FastQC (version 0.11.5)