FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68625826
Sequences flagged as poor quality0
Sequence length57
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2782880.4055149733279713No Hit
GTCTAGCACCGGCGACGGATCATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACG1539560.22434119772926303No Hit
GTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGTCTTCC1211320.17651080804477312No Hit
GGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGTGGTGCGATAGACCGCGA1210430.1763811192596793No Hit
GTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAGGGCAAAAGC917040.1336289926768969No Hit
TTCTTGGAGCGCGCGGAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCT799150.11645032877272764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC248600.035.9193881
CTAGCAC251800.035.7543833
GCACCGG250000.035.6241846
CGACGGA251950.035.3933813
GCGACGG253100.035.27286512
AGCACCG254300.035.1421245
CACCGGC254800.035.043227
GGCGACG254700.034.9809511
ACCGGCG258300.034.5683828
TCTAGCA258900.034.53022
GACGGAT261100.034.14348214
ACGGATC262500.034.02948815
CGGATCA273000.032.74901616
GGATCAT273250.032.748717
CCGGCGA284700.031.4077079
GATCATT291900.030.76685318
TGCCCTA293350.030.18831633
ATCATTC312000.029.0544619
AGTGTCT307750.028.8854827
GCCCTAT307600.028.78938134
Produced by FastQC (version 0.11.5)