FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n01_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n01_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37453434
Sequences flagged as poor quality0
Sequence length57
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGCACCGGCGACGGATCATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACG2395520.639599562486046No Hit
GTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGTCTTCC1918140.5121399549104095No Hit
GTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAGGGCAAAAGC1793100.478754498185667No Hit
TTCTTGGAGCGCGCGGAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCT1153040.307859621096426No Hit
GTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACA1062460.28367492283885104No Hit
GTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGT1036590.27676767903311617No Hit
CTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGT1013350.2705626405311726No Hit
GTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGT1003070.2678178988874558No Hit
GCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCGCGAGACCGATAGC880390.23506255794862496No Hit
GAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCA869270.23209353780483788No Hit
GGCGTGTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGA809810.2162178239784368No Hit
GTATTCGCCTACCGAGGTTCTTACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGA784340.20941737945844968No Hit
AGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGC778180.20777267045793452No Hit
GCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACT773450.20650976890396752No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAG763110.20374900736738855No Hit
GTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAA735210.1962997571864839No Hit
GTTCTAGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGT726120.1938727434178666No Hit
CTTTAAAAGCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGG700950.18715239836219025No Hit
GTATATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCG682950.1823464305035421No Hit
CTTCTGATCGAGGCTTCGTCCCCTAGCGGGTGTCAGGCCCTTGGCGGCGGCGGGTGC671390.17925993114543248No Hit
GCCTGGACCTGTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCG661920.1767314580553548No Hit
GTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCGGG650710.1737384080722745No Hit
CCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAAT645560.17236336726827237No Hit
GTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAAAAAAAAAAA642240.17147693319656618No Hit
GTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGTAATTTTCGTG619500.16540539380180733No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAA617730.1649328069623736No Hit
ATCAGATACCGTCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGT604010.1612695914612262No Hit
TCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAA592850.15828989138886437No Hit
GTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGAGTGTC590220.157587686085073No Hit
GCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAG586910.15670392199551048No Hit
GAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACGTTCTTCTG584320.1560123966202939No Hit
GGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTT573980.1532516350837149No Hit
ATCTAAGGCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGT557000.14871800540372346No Hit
TTCCGGGACTGTTCTCAGTCCCGAAGCGGCTCGGCGCGGTTTCGGTCGGGTGCTCCC552770.14758860295694115No Hit
GAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGC551230.14717742570681236No Hit
GTGTAATTTTCGTGCGAGGTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAG546110.14581039484924133No Hit
GAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACGTTCTTCTGATCGAGGCTTCGTCC530700.14169595236580979No Hit
GGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGTGGTGCGATAGACCGCGA528010.14097772716915624No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAAAGTA521930.13935437802579065No Hit
GTATGAATACAGACCGCGAAAGCGTGGCCTATCGATCCTTTTGAACTTGCGAGTTTC515290.13758150988237822No Hit
GTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACC514130.13727179195370978No Hit
ATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACGGTATTCGCCTACCGAGGTTCT513700.13715698272153096No Hit
GCTCTAGGGGCTGGGTCGGTCGGGCCGTGGCACGAAGCGGGGTGCGGCGCTGTACCG506850.13532804495310094No Hit
GTTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCG496090.13245514416648685No Hit
CTCTCGGCGTCTTCCCGGAGTCGGGTTGTTTGGGAATGCAGCCCAAAGCGGGTGGTA494860.13212673636281255No Hit
GTCTAGTAGCTGGTTCCCTTCGAAGTTTCCCTCAGGATAGCTGGCGCTCTGTCGCAG471600.12591635789658168No Hit
GCGCTGTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGT468140.12499254407486372No Hit
GTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCGGCCCGCTTCG459860.12278179885988558No Hit
CCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTC458630.1224533910562113No Hit
GAGATAGGGACATGTGCCCCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCC457740.12221576264542257No Hit
GCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA451070.12043488455557907No Hit
ATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGT440830.11770082284043701No Hit
TCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGTTAATTTGACGA438760.11714813653669247No Hit
ATGCAGGACTCATTCGAGGCCCTGTAATTGGAATGAGTACACTTTAAAAGCTTTAAC431580.11523108935752059No Hit
CTATAAACGATGCCAACTAGCGATCGGGGAGCGTTAATTTGACGACCTCCCCGGCAG388810.10381157572894384No Hit
GATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAG388600.10375550610392627No Hit
ATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCGGTCG385710.10298388126439888No Hit
TATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCGGTCGG385450.10291446172866285No Hit
CATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACT382440.10211079710341113No Hit
CCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGCGG381170.10177170937116208No Hit
GACATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTA380290.10153675094251706No Hit
GGTAAACTCCATCTAAGGCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGT375350.1002177797635325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGG379900.035.4500126
ACCGGCG380050.035.4024778
GTCTAGC382500.035.292191
CTAGCAC383150.035.2690963
CGGCGAC382500.035.00925410
GGCGACG382750.034.9904511
CGACGGA383550.034.94434713
GCGACGG383450.034.9400212
AGCACCG388600.034.8138245
TAGCACC392450.034.5567554
TCTAGCA400000.033.8159332
ACGGATC404150.033.2897815
GACGGAT405250.033.1740314
CACCGGC415700.032.341867
GGATCAT421050.032.1677717
CGGATCA422350.031.98230216
CCGGCGA420950.031.9385459
GATCATT449700.030.2172818
TGCCCTA444450.030.18872633
GTCTGCC452000.029.7583630
Produced by FastQC (version 0.11.5)