FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n01_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n01_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41137956
Sequences flagged as poor quality0
Sequence length57
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGCACCGGCGACGGATCATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACG3283880.7982603705444189No Hit
GTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGTCTTCC2515530.6114863849822777No Hit
GTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAGGGCAAAAGC1782910.43339780906956094No Hit
TTCTTGGAGCGCGCGGAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCT1754550.426503932280933No Hit
GTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGT1279610.3110533736775838No Hit
CTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGT1265700.3076720681017793No Hit
GTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACA1079590.2624316093876905No Hit
AGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGC991690.24106448069515168No Hit
GTATTCGCCTACCGAGGTTCTTACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGA963850.23429700785328275No Hit
GTTCTAGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGT930620.2262193094863537No Hit
GTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGT916800.22285988151671904No Hit
CTTTAAAAGCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGG906240.22029290905945836No Hit
CTTCTGATCGAGGCTTCGTCCCCTAGCGGGTGTCAGGCCCTTGGCGGCGGCGGGTGC887460.2157277819053528No Hit
GTATATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCG881600.21430330665918357No Hit
GTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCGGG873950.21244371013474758No Hit
GAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACGTTCTTCTG842840.20488135093537463No Hit
CCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAAT822960.20004883081697106No Hit
GCCTGGACCTGTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCG810010.19690088637364483No Hit
GAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACGTTCTTCTGATCGAGGCTTCGTCC784610.1907265397434914No Hit
GCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCGCGAGACCGATAGC775230.1884464070115686No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAG762230.18528630834259244No Hit
GGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTT761950.18521824467895293No Hit
GAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCA759550.18463484184775733No Hit
GGCGTGTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGA750220.18236686334148444No Hit
GTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGTAATTTTCGTG744710.18102746767486452No Hit
GCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACT728200.1770141423652648No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAAAGTA718990.1747753340005517No Hit
TTCCGGGACTGTTCTCAGTCCCGAAGCGGCTCGGCGCGGTTTCGGTCGGGTGCTCCC702300.1707182534786123No Hit
GCTCTAGGGGCTGGGTCGGTCGGGCCGTGGCACGAAGCGGGGTGCGGCGCTGTACCG690290.16779880847750434No Hit
GGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGTGGTGCGATAGACCGCGA677910.16478942220658702No Hit
ATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACGGTATTCGCCTACCGAGGTTCT677730.16474566699424736No Hit
GTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACC674390.16393376472083346No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAA674370.1639289030305735No Hit
GTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAA668490.16249956609414432No Hit
ATCAGATACCGTCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGT639570.15546956197823733No Hit
CCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTC615210.149548023241602No Hit
GCGCTGTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGT615200.149545592396472No Hit
GCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA580700.14115917669803527No Hit
GAGATAGGGACATGTGCCCCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCC580310.141064373737966No Hit
TCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAA580170.14103034190614622No Hit
GTGTAATTTTCGTGCGAGGTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAG574150.1395669731378973No Hit
ATGCAGGACTCATTCGAGGCCCTGTAATTGGAATGAGTACACTTTAAAAGCTTTAAC569710.1384876779001854No Hit
GAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGC542520.13187820999176525No Hit
GTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCGGCCCGCTTCG539190.1310687385634814No Hit
GTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGAGTGTC532600.12946681162282347No Hit
CTCTCGGCGTCTTCCCGGAGTCGGGTTGTTTGGGAATGCAGCCCAAAGCGGGTGGTA529270.12865734019453956No Hit
CATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACT507020.12324870978033037No Hit
GTCTAGTAGCTGGTTCCCTTCGAAGTTTCCCTCAGGATAGCTGGCGCTCTGTCGCAG502090.1220503031312494No Hit
ATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCGGTCG502080.12204787228611942No Hit
GTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTA500590.12168567636175215No Hit
TATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCGGTCGG496950.12080084873443882No Hit
GACATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTA494150.12012021209804397No Hit
CATTCGTACTGTGCCGTTAGAGGTGAAATTCTTGGATCGGCGCAAGACGAACGACTG493940.12006916435031434No Hit
ATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGT491210.11940554362982936No Hit
GTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTT487180.11842591304244675No Hit
CCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGCGG481190.11696983680958772No Hit
GAATAATGGAATAGGACCTCGGTTCTATTTTGTTGGTTTTCGGAGCGCGAGGTAATG477280.11601937636376489No Hit
GTTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCG470920.11447335886109655No Hit
ATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGGATTGGCTC460590.11196229584182549No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAAAGTATA458670.11149557357686901No Hit
TCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGTTAATTTGACGA457530.11121845723205111No Hit
GTATGAATACAGACCGCGAAAGCGTGGCCTATCGATCCTTTTGAACTTGCGAGTTTC451700.1098012745212718No Hit
GCGCTGGAGCGTCGGGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGTGGT448330.10898207971246797No Hit
CAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCG446350.10850077237673161No Hit
GATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAG428760.10422491579309386No Hit
CTCCAAAAGTATATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGG426990.1037946562050871No Hit
GCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAG424400.10316506731642185No Hit
ATCTAAGGCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGT414610.1007852699341698No Hit
GTACGGTATTCGCCTACCGAGGTTCTTACGGGTAACGGGGAATCAGGGTTCGATTCC413980.10063212669098096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC504450.035.59221
CTAGCAC509600.035.342943
GCACCGG507050.035.3346336
ACCGGCG507200.035.314268
CACCGGC508100.035.286837
CGGCGAC506550.035.2188510
GGCGACG508400.035.12029611
AGCACCG513700.035.016195
CGACGGA510250.034.99859613
GCGACGG511500.034.91792712
TAGCACC518750.034.8620764
TCTAGCA517800.034.6757162
GACGGAT520000.034.38181314
ACGGATC531450.033.69872315
GGATCAT552000.032.60872317
CGGATCA552950.032.48103316
CCGGCGA557600.032.1040849
GATCATT577750.031.19587318
TGCCCTA595000.029.97292333
ATCATTC611750.029.80785419
Produced by FastQC (version 0.11.5)