FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n01_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n01_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38688246
Sequences flagged as poor quality0
Sequence length57
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGCACCGGCGACGGATCATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACG2830480.7316123868732638No Hit
GTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGTCTTCC2176620.5626049834360545No Hit
GTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAGGGCAAAAGC1999130.5167280005405259No Hit
TTCTTGGAGCGCGCGGAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCT1360370.3516235913098774No Hit
CTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGT1089320.281563552919923No Hit
GTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGT1061120.27427451738184255No Hit
GTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACA1054740.2726254377104612No Hit
GTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGT1006380.26012551719196575No Hit
GCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCGCGAGACCGATAGC973050.2515104975294047No Hit
AGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGC970630.2508849845506049No Hit
GTATTCGCCTACCGAGGTTCTTACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGA922620.23847553078524159No Hit
GTTCTAGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGT873810.22585929586986186No Hit
GAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCA871240.225195011425434No Hit
CTTCTGATCGAGGCTTCGTCCCCTAGCGGGTGTCAGGCCCTTGGCGGCGGCGGGTGC870220.22493136545916298No Hit
GGCGTGTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGA832260.21511959989088159No Hit
GTATATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCG789550.20408007124437744No Hit
GTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCGGG779000.20135314482853528No Hit
GTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAA770800.19923363804086647No Hit
GCCTGGACCTGTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCG737920.19073493277518966No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAG727650.18808037976185327No Hit
CTTTAAAAGCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGG717920.18556540402477797No Hit
ATCAGATACCGTCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGT713640.18445912487218985No Hit
GTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGTAATTTTCGTG703340.18179681756572785No Hit
GAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACGTTCTTCTG683670.17671258603969794No Hit
GCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACT682790.1764851267746798No Hit
CCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAAT681230.17608190353214773No Hit
TTCCGGGACTGTTCTCAGTCCCGAAGCGGCTCGGCGCGGTTTCGGTCGGGTGCTCCC664900.1718609833074366No Hit
GAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACGTTCTTCTGATCGAGGCTTCGTCC610250.15773524599693664No Hit
GGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGTGGTGCGATAGACCGCGA606230.1566961707181039No Hit
ATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACGGTATTCGCCTACCGAGGTTCT602400.15570620596240006No Hit
GGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTT601000.15534433894987124No Hit
CTCTCGGCGTCTTCCCGGAGTCGGGTTGTTTGGGAATGCAGCCCAAAGCGGGTGGTA589960.152490759079644No Hit
GTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGAGTGTC588310.152064272957735No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAA584660.15112083396078488No Hit
GAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGC582470.15055477056261482No Hit
GCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAG575980.1488772584831062No Hit
ATCTAAGGCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGT572150.1478872937274024No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAAAGTA571380.14768826687051154No Hit
GTGTAATTTTCGTGCGAGGTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAG554450.14331226078328804No Hit
GTTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCG553010.1429400547132584No Hit
GTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACC551190.1424696275969709No Hit
TCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAA537510.13893366993168935No Hit
GTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCGGCCCGCTTCG530990.1372484035590551No Hit
CCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTC525590.13585263079644397No Hit
GTATGAATACAGACCGCGAAAGCGTGGCCTATCGATCCTTTTGAACTTGCGAGTTTC525170.13574407069268532No Hit
GTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAAAAAAAAAAA524080.13546233137578786No Hit
TCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGTTAATTTGACGA520180.1344542732694576No Hit
GCTCTAGGGGCTGGGTCGGTCGGGCCGTGGCACGAAGCGGGGTGCGGCGCTGTACCG519360.13424232259069072No Hit
GTCTAGTAGCTGGTTCCCTTCGAAGTTTCCCTCAGGATAGCTGGCGCTCTGTCGCAG511100.1321073072167707No Hit
GCGCTGTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGT496130.12823791494708756No Hit
ATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGT486530.12575654114688994No Hit
GCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA469620.12138570458841685No Hit
CCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGCGG466760.12064646197710799No Hit
GAGATAGGGACATGTGCCCCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCC460070.11891725461009527No Hit
CATTCGTACTGTGCCGTTAGAGGTGAAATTCTTGGATCGGCGCAAGACGAACGACTG459820.11885263550071513No Hit
CTATAAACGATGCCAACTAGCGATCGGGGAGCGTTAATTTGACGACCTCCCCGGCAG449500.1161851586655027No Hit
GATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAG441000.11398810894657774No Hit
ATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCGGTCG439140.11350734277278944No Hit
TATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCGGTCGG435970.11268797246584919No Hit
GTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTT433990.11217618911955843No Hit
GAATAATGGAATAGGACCTCGGTTCTATTTTGTTGGTTTTCGGAGCGCGAGGTAATG422230.10913650621431636No Hit
CCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAGGGCAAAAGCTC420270.108629892396776No Hit
ATGCAGGACTCATTCGAGGCCCTGTAATTGGAATGAGTACACTTTAAAAGCTTTAAC419080.10832230543612652No Hit
CAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCG414410.10711522047290539No Hit
GTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTA402600.10406261374578728No Hit
GAGTAGGAGGGCCGCGGCGGTGGGCGTCGAAGCCTAGGGCGCGAGCCCGGGTGGAGC389700.10072826770177175No Hit
CATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACT387820.10024233199923305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCG422150.036.1658338
GCACCGG421350.036.1558346
CACCGGC422250.036.1149987
GTCTAGC422650.036.0907361
CTAGCAC424550.036.0635763
CGGCGAC423350.035.9070110
AGCACCG428200.035.7322545
CGACGGA428550.035.6187113
GGCGACG428750.035.53648811
GCGACGG430150.035.49205812
TAGCACC433300.035.399954
TCTAGCA439450.034.8126452
GACGGAT442000.034.4601514
ACGGATC445000.034.2451615
CGGATCA457250.033.39515316
CCGGCGA462800.032.9672169
GGATCAT463250.032.95356817
GATCATT490000.031.21367818
TGCCCTA507000.029.8840633
ATCATTC519050.029.79100219
Produced by FastQC (version 0.11.5)