FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n01_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n01_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43297086
Sequences flagged as poor quality0
Sequence length57
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGCACCGGCGACGGATCATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACG3567720.8240092647343519No Hit
GTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGTCTTCC2579580.5957860535926136No Hit
TTCTTGGAGCGCGCGGAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCT2067630.47754483985365664No Hit
GTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAGGGCAAAAGC1807310.4174207012453448No Hit
GTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGT1360550.3142359280252717No Hit
CTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGT1300920.3004636385922138No Hit
GTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACA1153660.2664521118118665No Hit
AGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGC979450.22621614766407142No Hit
GTATTCGCCTACCGAGGTTCTTACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGA960300.22179321721558812No Hit
GTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGT926780.21405135671255104No Hit
GAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACGTTCTTCTG924250.21346702177601515No Hit
CTTCTGATCGAGGCTTCGTCCCCTAGCGGGTGTCAGGCCCTTGGCGGCGGCGGGTGC897020.20717791492942503No Hit
GTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGTAATTTTCGTG868660.20062782054201062No Hit
GTTCTAGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGT865750.19995571988378155No Hit
CTTTAAAAGCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGG852150.19681463089686912No Hit
CCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAAT839800.19396224494184205No Hit
GTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCGGG827510.19112371673234543No Hit
GCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCGCGAGACCGATAGC827150.1910405702591625No Hit
GAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACGTTCTTCTGATCGAGGCTTCGTCC808670.18677238463576973No Hit
GCCTGGACCTGTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCG806440.18625733842688627No Hit
GCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACT802670.18538661008272012No Hit
GTATATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCG798980.1845343587325946No Hit
GGCGTGTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGA798380.18439578127728964No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAG785400.18139788899419235No Hit
GGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTT760720.17569773633264835No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAA747570.17266058043721463No Hit
GAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCA736500.17010382638683813No Hit
TTCCGGGACTGTTCTCAGTCCCGAAGCGGCTCGGCGCGGTTTCGGTCGGGTGCTCCC719740.16623289613531958No Hit
GCTCTAGGGGCTGGGTCGGTCGGGCCGTGGCACGAAGCGGGGTGCGGCGCTGTACCG710870.16418425942106127No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAAAGTA695320.16059279370440774No Hit
GTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAA663490.1532412597004796No Hit
ATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACGGTATTCGCCTACCGAGGTTCT658460.15207951870017306No Hit
ATCAGATACCGTCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGT644120.1487675175183845No Hit
GTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACC637660.1472755002496011No Hit
GCGCTGTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGT624310.14419215186906573No Hit
GGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGTGGTGCGATAGACCGCGA616220.14232366584670386No Hit
CCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTC609850.1408524351962162No Hit
TCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAA588330.1358821237992783No Hit
GAGATAGGGACATGTGCCCCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCC586110.13536938721464994No Hit
GTGTAATTTTCGTGCGAGGTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAG579910.1339374201764987No Hit
GTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCGGCCCGCTTCG576850.13323067515444342No Hit
ATGCAGGACTCATTCGAGGCCCTGTAATTGGAATGAGTACACTTTAAAAGCTTTAAC564680.1304198624360078No Hit
CTCTCGGCGTCTTCCCGGAGTCGGGTTGTTTGGGAATGCAGCCCAAAGCGGGTGGTA556470.12852366092258496No Hit
GCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA555740.12835505835196392No Hit
GTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGAGTGTC545770.1260523629696465No Hit
GAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGC521770.12050926475744811No Hit
CATTCGTACTGTGCCGTTAGAGGTGAAATTCTTGGATCGGCGCAAGACGAACGACTG520810.12028754082896018No Hit
GAATAATGGAATAGGACCTCGGTTCTATTTTGTTGGTTTTCGGAGCGCGAGGTAATG513160.11852067827382194No Hit
GACATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTA511890.11822735599342644No Hit
CATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACT508220.1173797238918111No Hit
GTTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCG506500.11698246851993689No Hit
GTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTA496650.11470748862868047No Hit
ATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGGATTGGCTC481410.11118762126393449No Hit
GCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAG475670.10986189694151703No Hit
GTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTT474700.10963786338877402No Hit
ATCTAAGGCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGT468190.10813429799871521No Hit
GTCTAGTAGCTGGTTCCCTTCGAAGTTTCCCTCAGGATAGCTGGCGCTCTGTCGCAG461200.10651987064441241No Hit
CCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGCGG457480.10566069042152167No Hit
GTATGAATACAGACCGCGAAAGCGTGGCCTATCGATCCTTTTGAACTTGCGAGTTTC454560.10498628013903755No Hit
TCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGTTAATTTGACGA449490.10381530064171061No Hit
ATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCGGTCG448990.10369981942895648No Hit
ATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGT444320.10262122490183288No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAAAGTATA443620.1024595512039771No Hit
TATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCGGTCGG437930.10114537500283506No Hit
GGGCTGGGTCGGTCGGGCCGTGGCACGAAGCGGGGTGCGGCGCTGTACCGGACTGGG435170.10050791870843225No Hit
CAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCG434050.10024924079186298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCG532550.036.3619778
CACCGGC533650.036.287027
GTCTAGC534200.036.2651141
CTAGCAC537200.036.2513123
GCACCGG535600.036.2310336
CGGCGAC533750.036.10846710
GGCGACG537600.035.9118911
CGACGGA538800.035.8604713
AGCACCG543250.035.838165
GCGACGG543250.035.61827512
TAGCACC550200.035.4874154
TCTAGCA550450.035.30542
GACGGAT550050.035.11348314
ACGGATC558200.034.60096715
GGATCAT579100.033.46441717
CGGATCA583400.033.16354816
CCGGCGA587550.032.9972579
GATCATT604750.032.06624218
TGCCCTA618550.031.08194733
GTCTGCC627700.030.72264930
Produced by FastQC (version 0.11.5)