FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n01_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n01_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37366445
Sequences flagged as poor quality0
Sequence length57
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGCACCGGCGACGGATCATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACG2714860.7265502511678593No Hit
GTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGTCTTCC2022790.5413386261390399No Hit
GTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAGGGCAAAAGC1912960.5119459450852228No Hit
TTCTTGGAGCGCGCGGAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCT1219800.32644261449008594No Hit
GTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGT1069750.28628626565893545No Hit
GTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGT1051270.2813406520208171No Hit
GTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACA999180.26740033738826374No Hit
CTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGT966790.25873213253227595No Hit
GCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCGCGAGACCGATAGC954480.2554377329713865No Hit
AGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGC942730.25229320049044No Hit
GTATTCGCCTACCGAGGTTCTTACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGA884180.23662406204283015No Hit
GAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCA877560.2348524190620756No Hit
CTTCTGATCGAGGCTTCGTCCCCTAGCGGGTGTCAGGCCCTTGGCGGCGGCGGGTGC821330.21980415851708665No Hit
GTTCTAGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGT814560.21799237256849027No Hit
GTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAA789470.21127779214747347No Hit
GGCGTGTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGA785520.21022069399430424No Hit
GTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCGGG777020.2079459258165983No Hit
GTATATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCG725690.1942090022211104No Hit
GCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACT710100.19003680976341206No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAG705050.18868532984606912No Hit
CTTTAAAAGCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGG703170.18818220464911767No Hit
ATCAGATACCGTCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGT690360.184753995195422No Hit
GTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGTAATTTTCGTG665890.17820533904148494No Hit
GCCTGGACCTGTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCG663150.1774720608289068No Hit
GAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACGTTCTTCTG629080.16835425473309007No Hit
GGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTT620730.16611962952322598No Hit
CCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAAT599390.16040862329825598No Hit
GAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGC592410.15854063719468095No Hit
GCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAG585750.15675828942250192No Hit
GGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGTGGTGCGATAGACCGCGA580390.1553238473716191No Hit
ATCTAAGGCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGT572990.15334346095808687No Hit
GTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACC571620.15297682185179778No Hit
GTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAAAAAAAAAAA566210.15152899881163434No Hit
GTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGAGTGTC561130.15016949030072302No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAAAGTA555420.14864138132487584No Hit
CTCTCGGCGTCTTCCCGGAGTCGGGTTGTTTGGGAATGCAGCCCAAAGCGGGTGGTA554190.1483122089885725No Hit
GAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACGTTCTTCTGATCGAGGCTTCGTCC550680.14737286354107274No Hit
TTCCGGGACTGTTCTCAGTCCCGAAGCGGCTCGGCGCGGTTTCGGTCGGGTGCTCCC550060.147206939273993No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAA544710.14577517342096633No Hit
ATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACGGTATTCGCCTACCGAGGTTCT544550.14573235425526831No Hit
GTTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCG539530.14438890293149376No Hit
TCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAA539320.14433270277651514No Hit
GCTCTAGGGGCTGGGTCGGTCGGGCCGTGGCACGAAGCGGGGTGCGGCGCTGTACCG538560.14412931173944965No Hit
GTGTAATTTTCGTGCGAGGTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAG520560.13931215559842527No Hit
GTATGAATACAGACCGCGAAAGCGTGGCCTATCGATCCTTTTGAACTTGCGAGTTTC519360.1389910118556903No Hit
CCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTC514510.13769305589546985No Hit
GTCTAGTAGCTGGTTCCCTTCGAAGTTTCCCTCAGGATAGCTGGCGCTCTGTCGCAG498260.13334423437926726No Hit
TCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGTTAATTTGACGA495160.13251461304386863No Hit
ATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGT491170.1314468100992749No Hit
GTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCGGCCCGCTTCG488670.13077776063524374No Hit
GCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA453370.12133078220312368No Hit
CCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGCGG451440.12081427601689163No Hit
CTATAAACGATGCCAACTAGCGATCGGGGAGCGTTAATTTGACGACCTCCCCGGCAG444730.11901854725543197No Hit
GTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTT437720.1171425325582886No Hit
GAGATAGGGACATGTGCCCCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCC435280.11648954028139417No Hit
GCGCTGTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGT428150.11458141120997731No Hit
ATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCGGTCG427800.11448774428501293No Hit
ATGCAGGACTCATTCGAGGCCCTGTAATTGGAATGAGTACACTTTAAAAGCTTTAAC422840.1131603501483751No Hit
GTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTA421500.1128017396356544No Hit
CATTCGTACTGTGCCGTTAGAGGTGAAATTCTTGGATCGGCGCAAGACGAACGACTG420620.11256623422431543No Hit
GACATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTA418930.11211395678663036No Hit
CATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACT417320.1116830889317943No Hit
TATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCGGTCGG415900.11130306883622458No Hit
GATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAG411510.11012821797738587No Hit
CCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAGGGCAAAAGCTC410940.10997567469958676No Hit
CAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCG404750.10831910822664559No Hit
GAATAATGGAATAGGACCTCGGTTCTATTTTGTTGGTTTTCGGAGCGCGAGGTAATG380950.10194975732906891No Hit
GGTAAACTCCATCTAAGGCTAAATACAGTCGCGAGACCGATAGCGAACAAGTACCGT378340.10125126968862036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAC411500.036.289623
ACCGGCG409950.036.2712978
GCACCGG410550.036.218296
CACCGGC410450.036.1339347
GTCTAGC412800.036.043791
AGCACCG415950.035.913595
CGGCGAC413050.035.86983510
CGACGGA416000.035.676513
GGCGACG416800.035.5892411
TAGCACC420100.035.5588154
GCGACGG417850.035.53671312
TCTAGCA425350.035.0067332
ACGGATC434300.034.18522615
GACGGAT436700.034.01482814
CGGATCA451100.033.00863316
GGATCAT453100.032.8878217
CCGGCGA459550.032.412139
GATCATT483750.030.88923618
TGCCCTA487700.030.23528733
GTCTGCC492800.030.00035330
Produced by FastQC (version 0.11.5)