FastQCFastQC Report
Thu 18 Apr 2019
H7JC7BGXB_n01_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JC7BGXB_n01_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45794985
Sequences flagged as poor quality0
Sequence length57
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGGACTGTTCTCAGTCCCGAAGCGGCTCGGCGCGGTTTCGGTCGGGTGCTCCC4062330.887068747811578No Hit
GTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGTCTTCC2824480.6167662245112647No Hit
GTCTAGCACCGGCGACGGATCATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACG2667420.5824698927186023No Hit
GTACAGGCGCGGCGCGTGCAAGTGCCGCCGCCCGGTCGAGGAGAGATTTCGCCGCGT2651490.5789913458864546No Hit
GTTCTCAGTCCCGAAGCGGCTCGGCGCGGTTTCGGTCGGGTGCTCCCTCGCGAGGCG2065360.45100134872846886No Hit
GTCCCGAAGCGGCTCGGCGCGGTTTCGGTCGGGTGCTCCCTCGCGAGGCGCTGCGGG1698950.3709904042986366No Hit
GTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAGGGCAAAAGC1618180.35335310187349117No Hit
GTCTAGTAGCTGGTTCCCTTCGAAGTTTCCCTCAGGATAGCTGGCGCTCTGTCGCAG1487770.32487618458658735No Hit
GTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGT1479950.3231685740261734No Hit
CTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGT1347580.29426366227655715No Hit
GTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACA1183520.25843877883135025No Hit
GTCGAGGAGAGATTTCGCCGCGTCTGTCCCGCTTCGGCGGGGCCGGGGAGTCCGGCC1179260.25750854596851597No Hit
GCCTTTGGTCGGGTGGTACAGGCGCGGCGCGTGCAAGTGCCGCCGCCCGGTCGAGGA1148390.25076763318079476No Hit
GGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGTGGTGCGATAGACCGCGA1127810.246273691322314No Hit
AGGTAGCCCGGCCTTTGGTCGGGTGGTACAGGCGCGGCGCGTGCAAGTGCCGCCGCC1094930.23909386584579076No Hit
CGTCTGTCCCGCTTCGGCGGGGCCGGGGAGTCCGGCCGAGCTGCGTCGGGTCGTTCC995930.21747577818837585No Hit
GCGTCGGGTCGTTCCGGGACTGTTCTCAGTCCCGAAGCGGCTCGGCGCGGTTTCGGT988250.21579873866101276No Hit
GTTTCGGTCGGGTGCTCCCTCGCGAGGCGCTGCGGGTCCGCGGCTAAAGTCAGCCAC980090.21401688416318948No Hit
AGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGC966480.21104494302159943No Hit
TTCTTGGAGCGCGCGGAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCT961400.2099356512508957No Hit
GTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGTAATTTTCGTG934740.20411405309991912No Hit
GCCTGGACCTGTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCG919380.20075997404519294No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAA910930.19891479383604996No Hit
CCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAAT887300.19375484018610334No Hit
GGCGCGGCGCGTGCAAGTGCCGCCGCCCGGTCGAGGAGAGATTTCGCCGCGTCTGTC875810.19124583183071248No Hit
GCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACT871650.19033743542005746No Hit
GTTCTAGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGT858810.18753363496024728No Hit
GTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGT842510.18397429325503656No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAG826390.18045425716374838No Hit
GGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTT820550.1791790083564827No Hit
GTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCGGG805020.17578780733305185No Hit
CCGTAGAGCTGTACGAAACTCGAAGGCGAAATGAAAGTGAATGCCGGCGAGGTCCGG797300.17410203322481707No Hit
GCTCTAGGGGCTGGGTCGGTCGGGCCGTGGCACGAAGCGGGGTGCGGCGCTGTACCG770690.1682913532999301No Hit
GAATAATGGAATAGGACCTCGGTTCTATTTTGTTGGTTTTCGGAGCGCGAGGTAATG762780.16656408993255484No Hit
TTCCCGACCCGTCTTGAAACACGGACCAAGGAGTCTAACATGTGCGCGAGCCGTAGA758260.16557708229405468No Hit
CATTCGTACTGTGCCGTTAGAGGTGAAATTCTTGGATCGGCGCAAGACGAACGACTG758000.16552030751838875No Hit
ATCAGATACCGTCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGT754470.16474948075646273No Hit
GATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAAC753430.16452238165379898No Hit
GCGCTGGAGCGTCGGGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGTGGT749120.16358123056487517No Hit
GTCTTGAAACACGGACCAAGGAGTCTAACATGTGCGCGAGCCGTAGAGCTGTACGAA735930.16070100252243777No Hit
GGCGTGTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGA728710.15912441067509903No Hit
GCTGGGAGGCGGACAAGCTGCGGCGCGCAAGGTAGCCCGGCCTTTGGTCGGGTGGTA717440.15666344251450243No Hit
GCTAAAGTCAGCCACCTTCCCGACCCGTCTTGAAACACGGACCAAGGAGTCTAACAT697740.15236166143519864No Hit
GAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCA695430.15185723938985896No Hit
GTATTCGCCTACCGAGGTTCTTACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGA694730.1517043842246045No Hit
GCGCTGTACCGGACTGGGCGAACGCCTTCGGGTGTGAGCCTGGACCTGTGTCGGCGT694300.15161048748023392No Hit
GAGATAGGGACATGTGCCCCGTAAAGCAGCACGTCTCTTGTGCTGTCCGGAGAGCCC680640.14862762811255426No Hit
TCTCAGTCCCGAAGCGGCTCGGCGCGGTTTCGGTCGGGTGCTCCCTCGCGAGGCGCT680390.14857303698210622No Hit
GTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACC679230.14831973413682742No Hit
GTCTAACATGTGCGCGAGCCGTAGAGCTGTACGAAACTCGAAGGCGAAATGAAAGTG677620.1479681672567422No Hit
GAGTTAAACAGTACGTGAAACCGTCGAGAGGCAAACGGGAGGGCCTGTCGGGTCTGC652480.14247848317888956No Hit
CTTTAAAAGCTTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGG651910.14235401540146808No Hit
GTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCGGCCCGCTTCG651160.14219024201012403No Hit
GCGCAAGGTAGCCCGGCCTTTGGTCGGGTGGTACAGGCGCGGCGCGTGCAAGTGCCG644710.14078179084456519No Hit
GGGCCGGGGAGTCCGGCCGAGCTGCGTCGGGTCGTTCCGGGACTGTTCTCAGTCCCG638290.1393798906146601No Hit
TCTCCAAGGTGAACAGCCTCTGACCGATAGAACAATGTAGGTAAGGGAAGTCGGCAA632600.13813739648566323No Hit
GTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAA620840.13556942970938848No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAATCAGCCCTGAAAATGGATGGC610040.13321109287403415No Hit
CCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTC608680.1329141171243969No Hit
GTGTAATTTTCGTGCGAGGTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAG602310.13152313512058142No Hit
GAGTAGGAGGGCCGCGGCGGTGGGCGTCGAAGCCTAGGGCGCGAGCCCGGGTGGAGC596520.13025880453940536No Hit
GTATATATTTAAGTTGTTGCGGTTGAAAAGCTCGTAGTTGGATTTTGGGCGCGGGCG593960.12969979136361764No Hit
CCCTCGCGAGGCGCTGCGGGTCCGCGGCTAAAGTCAGCCACCTTCCCGACCCGTCTT587040.12818870887281653No Hit
ATGTAGGTAAGGGAAGTCGGCAAACTAGATCCGTAACTTCGGGAAAAGGATTGGCTC567170.1238498058248081No Hit
GACATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTA553580.12088223197365389No Hit
CATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATACCACTACT549030.11988867339949996No Hit
GCCGGGGAGTCCGGCCGAGCTGCGTCGGGTCGTTCCGGGACTGTTCTCAGTCCCGAA542920.11855446617135043No Hit
GGACTGTTCTCAGTCCCGAAGCGGCTCGGCGCGGTTTCGGTCGGGTGCTCCCTCGCG539690.11784914876596204No Hit
GAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGC533900.11658481818478596No Hit
GTCTTCGGGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTA522030.11399283131111408No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAAAGTA520710.11370459014234854No Hit
GCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACAGTCGCGAGACCGATAGC514140.11226993523417465No Hit
TCCTAGTTCTGACTATAAACGATGCCAACTAGCGATCGGGGAGCGTTAATTTGACGA510770.11153404679573539No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAATCAGCCCTGAAAATGGATG508610.11106237942866452No Hit
ATGGTGAACTATGCCTGAGGAGGTTGAAGCCAGAGGAAACTCTGGTGGAGGACCGTA507870.11090078968253839No Hit
GATTAAGAGGGACAGACGGGGGCATTCGTACTGTGCCGTTAGAGGTGAAATTCTTGG505190.11031557276413564No Hit
GGGCTGGGTCGGTCGGGCCGTGGCACGAAGCGGGGTGCGGCGCTGTACCGGACTGGG500350.10925868847866202No Hit
GTGTAACAACTCACCTGCCGAATCAATCAGCCCTGAAAATGGATGGCGCTGGAGCGT496450.10840706684367295No Hit
GTGGAGGGCCGCGACCGGCTGGGAGGCGGACAAGCTGCGGCGCGCAAGGTAGCCCGG496410.10839833226280125No Hit
ATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGT494800.10804676538271603No Hit
GCGCGGTTTCGGTCGGGTGCTCCCTCGCGAGGCGCTGCGGGTCCGCGGCTAAAGTCA485670.10605309729875444No Hit
GTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTT478490.10448524003228737No Hit
GTCTAACATGTGCGCGAGCCGTAGAGCTGTACGAAACTCGAAGGCAAAAAAAAAAAA476860.1041293058617663No Hit
GAATAATGGTGCATGGAATAATGGAATAGGACCTCGGTTCTATTTTGTTGGTTTTCG476750.10410528576436917No Hit
ATTCAAGTGTCTGCCCTATCAACTTTCGTCGGTACGGTATTCGCCTACCGAGGTTCT471780.10302001409106258No Hit
GGGTGGTACAGGCGCGGCGCGTGCAAGTGCCGCCGCCCGGTCGAGGAGAGATTTCGC465430.10163339937768294No Hit
CTCTGGTGGAGGACCGTAGCGATTCTGACGTGCAAATCGATCGTCAAATTTGGGTAT461670.10081234877574476No Hit
GTCCCGCTTCGGCGGGGCCGGGGAGTCCGGCCGAGCTGCGTCGGGTCGTTCCGGGAC460400.10053502583306884No Hit
GTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGACTGAGTGTC458120.10003715472338291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGGC415550.036.23747
CTAGCAC420150.036.0773243
GCACCGG417900.036.070196
GTCTAGC418900.036.0117871
AGCACCG423800.035.7485245
CGACGGA419450.035.74032213
CGGCGAC421250.035.60207410
GGCGACG421500.035.5903911
GCGACGG421700.035.5737812
TAGCACC427050.035.5122874
ACCGGCG426950.035.3355188
TCTAGCA430050.035.1408162
ACGGATC430700.034.8959315
GACGGAT431100.034.7983714
GGATCAT449900.033.5620117
CGGATCA463400.032.53839516
GGTATCA121350.032.0345731
TGCCCTA471800.031.64961433
GATCATT483250.031.31176818
GCCCTAT481350.031.03180534
Produced by FastQC (version 0.11.5)