FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28051649
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1808620.6447464104516637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTG16250.023.6770218-19
GTACGAC29200.021.47346342-43
TGTTTCG33050.020.48266828-29
CGGTTCG6400.020.39936412-13
CCGATGT33750.020.0554224-25
GGCCGAT33400.019.8384222-23
TTTCGCA32650.019.42341230-31
ATCGGCG33250.019.14114236-37
CGTACGA28300.017.87615842-43
TCGCATC36400.017.35579532-33
ACGTGCT35850.017.09450562-63
TACGACT30550.016.71440944-45
AAGGGGG99350.016.47789682-83
CATTCGC15200.016.4133182-83
CGCATCG32400.016.27189634-35
GCATTCG31000.016.0956382-83
ACGACTG32350.016.00464244-45
TTCGCAT32650.015.78486132-33
TCGGAAG132850.015.3551893
AAAAGGG108200.015.26103880-81
Produced by FastQC (version 0.11.5)