FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22715465
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG105800.014.43743350-51
AAGGGGG278400.014.24286282-83
AAAAGGG309750.012.47059880-81
GGGGGGA363950.011.76568520-21
AAAGGGG286100.010.16346280-81
TACCGTA21550.010.1344329
CGGGGAT71450.010.0260241
AGGGGGG343650.09.24326182-83
CCGGGAT67550.08.9193341
GGTTTTT257100.08.73873242-43
CGCAAAT30550.08.5409371
TCGGATT37800.08.5343642
GGGGGAA347700.08.31966420-21
GGGGAAA330650.08.27414622-23
TTTAGGG132450.08.21390948-49
CGGGGTT76100.07.9172271
AAAAAAG594650.07.78657978-79
CGCGAGT22550.07.7841151
CCGGAAT53700.07.77432061
CGACCGT18950.07.76080041
Produced by FastQC (version 0.11.5)