FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm329.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm329.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20737136
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG97600.015.01823850-51
AAGGGGG262750.014.93779982-83
AAAAGGG285650.013.74798580-81
GGGGGGA337300.012.14567420-21
CGCGATT6754.838512E-911.2447431
CGGGGAT63550.010.9732471
AAAGGGG267200.010.17205982-83
AGGGGGG313050.010.02567684-85
GGGGAAA310800.09.64268222-23
GGGGGAA332400.09.04527120-21
GGTTTTT244550.08.89565142-43
AGAATCG54500.08.88163953
CGGCGAT40150.08.8615371
TACCGTA20600.08.7580859
CGGGAAT51050.08.7350511
CGGGGTT70250.08.7111751
GGGAAAA299700.08.70824122-23
CCGGGAT59450.08.5381791
GAATCGC42450.08.4971144
CGAATCG12854.7293724E-118.4940383
Produced by FastQC (version 0.11.5)