FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20807867
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG274000.015.04375782-83
TAGGGGG94750.013.73977150-51
AAAAGGG297750.013.72333180-81
GGGGGGA350850.011.85278820-21
AAAGGGG273500.010.44998682-83
AGGGGGG335350.09.88292384-85
TACCGTA18550.09.7261539
CGCGGGT39500.09.489111
GGGGAAA315650.09.366878522-23
CGGGGAT69250.09.0437741
GGGGGAA326850.08.99555620-21
CGCGGAT27600.08.9390171
AAAAAAG557900.08.49560878-79
CCGGGAT59400.08.386841
GGGAAAA301200.08.3024222-23
GGCGAAT26600.08.2048691
GCGGAAT35000.08.1335231
CGGGGTT77650.07.88213251
CTACCGT26650.07.8387258
CGACAAC35750.07.8301751
Produced by FastQC (version 0.11.5)