FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21739246
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG279900.014.08171682-83
AAAAGGG298000.013.1061580-81
TAGGGGG99000.012.23808950-51
TACCGTA19400.011.9914269
CGCGTAT4851.3589979E-611.738571
CGCGGGT45300.011.4157371
GGGGGGA350700.011.0452320-21
CGACCGT17400.010.63386251
CGGGGAT67850.09.9991221
AAAGGGG276500.09.31421582-83
AGGGGGG344300.09.06031584-85
CCGGGAT64100.08.5856991
CTACCGT28450.08.3436158
GGGGGAA333050.08.28615820-21
CTCGGTT41000.08.2158071
AAAAAAG578900.08.09730778-79
GGGGAAA324750.08.08049922-23
CGGGGTT73700.07.917961
GCGGAAT39550.07.91722771
CGACCTA12058.987627E-97.87759264
Produced by FastQC (version 0.11.5)