FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22504509
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG262650.012.85383482-83
AAAAGGG289500.011.611649580-81
TAGGGGG91750.011.41852150-51
CGCGGGT46800.011.1513111
GGGGGGA324900.010.679543520-21
CGGGGAT70550.09.5492741
CGCGTAT5002.7915608E-49.4887521
CGGGCAT95650.08.7794511
AGGGGGG322650.08.54171884-85
AAAGGGG271050.08.44974682-83
CGGGGTT78800.08.248471
CGAATCG15650.08.1875133
GCGGAAT37700.08.1799581
GCGGGTT42950.08.1742451
CGGGTCT87850.08.1571373
GTCGATT18900.08.0328051
CGTATCG24850.08.0209453
GGGGAAA307250.07.945629622-23
GGGGGAA316850.07.915209320-21
CTCGGTT42600.07.7959221
Produced by FastQC (version 0.11.5)