FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38188794
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG551450.015.803369582-83
TAGGGGG187850.015.28977950-51
AAAAGGG575800.015.10516680-81
GGGGGGA711400.012.23550120-21
AAAGGGG538050.011.44560882-83
CGCGGGT78200.010.5552121
AGGGGGG687400.010.43503884-85
GGGGAAA607450.010.28972922-23
GGGGGAA654150.010.00606920-21
CGGGGAT126850.09.9101421
AAAAAAG1064100.09.75054178-79
CCGGGAT117400.09.6976761
GGGAAAA577100.09.53873922-23
GGCGAAT52900.08.9674471
AAAAAGG765350.08.88707380-81
TACCGTA36800.08.3858449
CGGCGAT66400.07.93011331
TAAAAAA1064350.07.888857470-71
CTACCGT47750.07.6557718
CGGGAAT88100.07.6460461
Produced by FastQC (version 0.11.5)