FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34645997
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG412450.014.7928882-83
TAGGGGG145350.014.18727150-51
AAAAGGG506900.012.11522680-81
CGCACTT71000.011.55890751
GGGGGGA539500.011.27390120-21
AGGGGGG474050.010.3584-85
TACCGTA27900.010.2101699
AAAGGGG450150.09.70629682-83
GGCGAAT80450.09.1987241
CGCGGGT72150.09.1391861
AATCCGC52350.09.0669915
GGGGAAA550350.08.966639522-23
CCGGGAT79350.08.9077581
GGGGGTT353750.08.88865440-41
TTTAGGG180100.08.86365948-49
GGGGGAA556350.08.85353620-21
GGTTTTT377750.08.77676642-43
CGGGGTT122050.08.7064121
GCGAGTT100450.08.4061881
CGGGAAT66700.08.3923821
Produced by FastQC (version 0.11.5)