FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm323.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm323.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21388426
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT3858.213465E-814.786581
AAGGGGG238300.014.22751782-83
CGCGATT7105.456968E-1212.6952861
TAGGGGG81600.011.82014750-51
GGGGGGA316400.011.61198920-21
CGCACTT40950.011.237381
AAAAGGG297750.011.05841480-81
CGCGAAT9205.456968E-1210.828761
AGGGGGG273450.09.90503782-83
CGCGGGT44300.09.63797951
CTCGTAT16550.09.4593911
AAAGGGG266600.09.36534980-81
CCGGGAT50700.09.07634451
GCGGGTT32600.09.0223851
GCGAGTT58400.08.9356691
CGGGGAT92700.08.751121
GGGGGTT219400.08.68124240-41
CGGGCAT73650.08.631361
CGACCGT12101.2369128E-108.6255051
TCGAACG20900.08.6252232
Produced by FastQC (version 0.11.5)