FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21414869
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG277850.011.09897282-83
CGCGAAA12600.010.5426861
AAAGGGG237800.010.40988480-81
GGGGGGA292650.09.90871820-21
CGGGGAT69750.09.8625131
AAGGGGG237600.09.7693782-83
TACCGTA19550.09.7143319
CGCGGGT47100.09.4682721
CGACGAT19300.08.6034881
CGGGAAT47150.08.4520371
CCGGGAT59100.08.3485231
GGGTTAA64550.08.24284766-67
AAAAGGG276450.08.05217180-81
CGCGAGT21800.08.0520971
GCGGGAT43250.07.7881811
GCGAAAG59850.07.7682951
GCGGGTT39900.07.728661
CTACCGT24650.07.7043438
CGGGGTT72300.07.677351
AATCTAG55050.07.58791545
Produced by FastQC (version 0.11.5)