FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20612782
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG254550.014.34605382-83
TAGGGGG91100.013.43034750-51
AAAAGGG284400.012.83109580-81
CGCGGGT42100.011.3809641
GGGGGGA314250.011.30583920-21
CGCGAAA12050.011.0232771
AAAGGGG248950.010.17364982-83
AGGGGGG299800.09.8589284-85
CGGGGAT73200.09.7212021
GGGGGTT195750.09.63270640-41
GGGGGAA313850.08.92150420-21
ACGATCG9601.2891178E-88.8980083
GGGGAAA317800.08.81744922-23
GGGGTTT224650.08.80578640-41
GGGAAAA301250.08.38743922-23
CGGGATT38350.08.287991
CGCGATT7452.4199679E-58.2780271
GGTTTTT224550.08.2387542-43
CGGGGTT71450.07.96743971
AAAAAAG562600.07.935224578-79
Produced by FastQC (version 0.11.5)