FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22140810
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG249600.013.56473482-83
TAGGGGG87900.011.97277850-51
AAAAGGG287100.011.93300980-81
GGGGGGA306200.011.24644320-21
CGGGGAT68050.010.1786181
TACCGTA21200.010.0773649
AGGGGGG274650.09.92262984-85
CGCGGGT42850.09.521641
AAAGGGG258600.09.17863382-83
GGGGAAA312650.08.85647922-23
GGGGGAA323000.08.73506720-21
CTACCGT28850.08.7215068
CGAATCG12653.45608E-118.6283153
CCGGGAT64300.08.48498251
CGACCGT17650.08.332611
GCGGAAT37300.08.2673931
GGGAAAA307450.08.23386422-23
CGGGAAT56200.08.1039921
GTATCGA30550.08.0783934
GCGGGAT49500.08.0507841
Produced by FastQC (version 0.11.5)