FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22331805
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG257700.013.77345482-83
TAGGGGG93050.012.0759950-51
AAAAGGG295750.011.92043180-81
GGGGGGA325850.011.23161120-21
CGCGGGT37950.010.125941
AGGGGGG293450.09.71547684-85
CGGGGAT69850.09.1691591
TACCGTA21950.09.0847229
AAAGGGG272450.08.667747582-83
GGGGAAA327150.08.66739822-23
TTGGGGG296050.08.6342650-51
CGGGAAT49750.08.4870951
GGGGGAA338250.08.3904920-21
TTTTGGG276500.08.33342348-49
CCGGAAT49550.08.2341151
GTCGATT17550.08.1097281
CCGGGAT62100.08.0979741
GGGAAAA313200.07.931244422-23
TTTGGGG294200.07.727091348-49
TTTAGGG124550.07.724281348-49
Produced by FastQC (version 0.11.5)