FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31560395
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG686530.21752896311975817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA297800.030.31025366-67
TTTAGGG417900.028.91602748-49
AAAAGGG2064550.028.30419580-81
TAGGGGG575400.027.65791150-51
AAGGGGG2176750.027.57615982-83
TTGGGGT742100.026.75272638-39
GTTAAAA493350.025.34400668-69
AAAGGGG2041950.023.98808180-81
GGTTTTT1391900.023.87191842-43
GGGAATA340150.023.79849264-65
TAAAAAA2775850.023.6681270-71
GGGGAAA1639350.023.65610322-23
GGATTAA261650.023.44954166-67
TTAGGGG445400.023.35468348-49
AAAAAAG2850650.023.23880678-79
GGGAATT332300.022.77381364-65
GGAATTA274900.022.70758464-65
GGGGAAT573050.022.59788562-63
AAAAAGG2258400.022.52655878-79
GGGAAAA1326000.022.478322-23
Produced by FastQC (version 0.11.5)