FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n02_AHm317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n02_AHm317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21770071
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG280800.016.37178682-83
TAGGGGG96850.015.40372750-51
AAAAGGG319850.014.587584580-81
GGGGGGA380050.012.16076720-21
AAAGGGG287700.011.48681382-83
AGGGGGG332700.011.48304284-85
CGCGGGT43950.011.1178191
CGGGGAT68950.010.8708651
GGGGAAA346850.010.28401322-23
TTGGGGG305750.010.10826450-51
TTTAGGG125350.010.06260648-49
GGGAAAA331300.09.98536722-23
GGGGGAA367000.09.97877420-21
TTTTGGG282800.09.74358748-49
AAAAAAG588550.09.3165578-79
GGTTTTT275500.09.06885542-43
TTTGGGG301700.08.96785748-49
CCGGGAT66000.08.91291
AAAAAGG425150.08.54943780-81
TGGGGGG389200.08.51465750-51
Produced by FastQC (version 0.11.5)