FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28051649
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1809160.6449389125038603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC191500.057.092278
ATCGGAA239800.046.6402052
TCGGAAG237700.046.4741253
CGGAAGA239650.046.2926374
GATCGGA239100.045.741351
ACGTCTG144950.036.9522614-15
CGTCTGA146850.036.60962716-17
CACGTCT147600.036.49691814-15
ACACGTC151400.035.85909712-13
CACACGT153650.035.48774712-13
AATCGGG43500.035.24423240-41
GCACACG161500.033.52725210-11
TGAACTC168850.031.81510420-21
CTGAACT170800.031.57992618-19
GAACTCC168300.031.4128720-21
CTCCAGT151050.030.90816324-25
GAATCGG44300.030.26840640-41
CCGGAGA61700.030.1171634-35
AGCACAC188350.029.83902710-11
AACTCCA182100.029.81735222-23
Produced by FastQC (version 0.11.5)