FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22715465
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT737030.32446177086843697TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT480820.21167077143259008TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG205400.037.1256840-41
AATCGGG203650.037.05988340-41
AGAATCG233200.033.8906838-39
CACTCCG246500.033.62262330-31
GAGCACA514350.032.567519
ATCGGAA517200.032.0415652
AGAGCAC523750.031.892328
TCGGAAG525050.031.6697433
CGGAAGA535950.031.0252424
GATCGGA535200.030.9100421
ACTCCGG270300.030.71492232-33
ATCGGGG232100.030.20511842-43
CGGAGAA277600.029.97552336-37
CCGGAGA279250.029.67951234-35
TCCGGAG294250.028.27145834-35
CTCCGGA300700.027.73608232-33
GTCACTC301800.027.64261428-29
AAGAGCA613750.027.2930327
ACGTCTG311900.026.74672114-15
ACACGTC318100.026.17315312-13
Produced by FastQC (version 0.11.5)