FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm329.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm329.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20737136
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1463630.7058014182864982TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG182250.042.03749544-45
CGTATGC183800.041.9424246-47
TATGCCG184000.041.91089648-49
CTCGTAT184050.040.96829644-45
TACTCGA200750.038.49353836-37
TCTCGTA194100.038.06336242-43
ACTCGAT201550.037.39813636-37
ATCTCGT201150.036.9772342-43
TTACTCG209650.036.62150234-35
ATGCCGT224650.034.20033648-49
GTATGCC227500.033.92754446-47
GAGCACA465400.033.6350489
CCGTCTT232550.033.2990552-53
CTCGATC227150.032.86989238-39
GCCGTCT235600.032.5722950-51
ATCGGAA474500.032.4516142
AGAGCAC483000.032.429098
TCGATCT231200.031.99622238-39
TCGGAAG484600.031.9112433
CGGAAGA494500.031.3585154
Produced by FastQC (version 0.11.5)