FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20807867
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1545240.7426229704370948TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC188400.042.09028646-47
TATGCCG188550.042.05851748-49
TCGTATG186400.041.98079344-45
CTCGTAT187500.041.37985644-45
TCTCGTA200550.037.7630242-43
GTCACGA214800.036.88179428-29
ATCTCGT212450.036.03903642-43
AGTCACG222750.035.98125528-29
ACGAGAT230950.034.77603532-33
ATGCCGT227350.034.5464448-49
CGAGATT230200.034.48700732-33
TCACGAG235700.034.07516530-31
GTATGCC231450.033.9638746-47
GAGCACA476000.033.7841959
AGAGCAC478650.033.5376178
CCGTCTT237200.033.32629452-53
GCCGTCT236150.033.09976650-51
ATCGGAA478250.033.0924952
CACGAGA241550.032.89597730-31
TCGGAAG485600.032.6776773
Produced by FastQC (version 0.11.5)