FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21739246
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1606600.739032071305509TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG190900.042.197944-45
TATGCCG193300.041.94643848-49
CGTATGC194600.041.72599446-47
CTCGTAT196600.040.8415844-45
TATCTCG202550.039.33613640-41
TCTCGTA207650.038.38158842-43
ATCTCGT222100.036.13038342-43
ACCGCTC230400.035.2714232-33
ATGCCGT236050.034.20883648-49
GTATGCC239250.033.97859246-47
ATCGGAA486950.033.232592
GAGCACA494800.033.153789
CGCTCAT246000.033.02527234-35
GCCGTCT245700.032.96336750-51
TCGGAAG495050.032.8790663
CCGTCTT248100.032.7525952-53
AGAGCAC503050.032.6478278
GTCACCG249950.032.4743828-29
GATCGGA502500.032.137671
CACCGCT254000.032.0596130-31
Produced by FastQC (version 0.11.5)