FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22504509
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT620060.27552700661009755TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT400060.17776881957300203TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG173250.036.0779740-41
AATCGGG170050.035.97482340-41
AGAATCG199800.032.11555538-39
CACTCCG214400.031.85588530-31
ACTCCGG234700.029.2929132-33
GAGCACA493100.028.0956559
ATCGGGG202450.028.03589242-43
CGGAGAA247550.027.83938236-37
AGAGCAC498650.027.7162768
ATCGGAA491150.027.6196062
TCGGAAG500400.027.2411523
CCGGAGA253400.027.16871634-35
GATCGGA511650.026.4835031
CGGAAGA516050.026.4697174
TCCGGAG262600.026.29827134-35
CTCCGGA265700.025.84840832-33
GTCACTC276350.024.92072728-29
ACGTCTG282550.024.28063214-15
AAGAGCA589850.023.4550657
CGTCTGA297000.023.43511616-17
Produced by FastQC (version 0.11.5)