FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38188794
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3327820.8714126976620419TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC408850.042.75760346-47
TCGTATG406100.042.71333344-45
TATGCCG412900.042.35726548-49
CTCGTAT406250.041.9025444-45
TACTCGA440000.039.6482736-37
ACTCGAT436400.039.09377336-37
TCTCGTA428250.039.06184842-43
TTACTCG454450.038.22036434-35
ATCTCGT442350.037.96706842-43
GAGCACA958800.036.7939649
AGAGCAC977700.036.0195478
GTATGCC485450.035.94231446-47
ATGCCGT488100.035.66111448-49
ATCGGAA985300.035.3732452
CCGTCTT502850.034.8801252-53
TCGGAAG1001100.034.8610843
GCCGTCT504950.034.435950-51
GATCGGA1017650.034.1924251
CTCGATC492750.034.09786638-39
CGGAAGA1027450.034.0545964
Produced by FastQC (version 0.11.5)