FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34645997
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2327680.6718467360024305TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG278550.042.69693444-45
CGTATGC282400.042.52791646-47
TATGCCG283900.042.2803548-49
CTCGTAT279800.041.89507344-45
TCTCGTA291850.039.36713842-43
GTCACGA317300.037.86226728-29
ATCTCGT306600.037.49648742-43
AGTCACG333000.036.2982428-29
ACGAGAT338050.035.74948532-33
GTATGCC342950.034.97779546-47
GCCGTCT343350.034.6435250-51
CGAGATT349750.034.5535832-33
CACGAGA352250.034.180130-31
TCACGAG358350.034.00913630-31
TGCCGTC363000.033.16077850-51
CATCTCG345850.033.08981340-41
GAGCACA747450.032.5003939
ATGCCGT370700.032.252248-49
CCGTCTT376900.031.94127552-53
ATCGGAA818250.029.3303052
Produced by FastQC (version 0.11.5)