FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm323.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm323.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21388426
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1302440.6089461655570166TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG158950.042.52189644-45
TATGCCG160800.042.24221848-49
CGTATGC161100.042.20625346-47
CTCGTAT161700.041.75467344-45
TATCTCG167950.039.9031540-41
TCTCGTA171750.038.99249342-43
ATCTCGT185400.036.41629442-43
ACCGCTC192600.035.60909332-33
GCCGTCT196900.034.24630750-51
GTATGCC199000.034.1560446-47
CGCTCAT201300.034.08225634-35
GTCACCG204150.033.44250528-29
TGCCGTC210900.032.24325650-51
CCGCTCA215350.031.7480332-33
GAGCACA440350.031.5361049
ATGCCGT214850.031.51581848-49
CCGTCTT221000.030.95518752-53
TCACCGC229100.030.01859930-31
ATCGGAA479000.028.5966952
AGAGCAC491100.028.267518
Produced by FastQC (version 0.11.5)