FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21414869
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT598750.2795954530471328TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT394990.1844466104368885TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG165500.035.91660340-41
GAATCGG169000.035.4257140-41
CACTCCG211900.031.1217630-31
AGAATCG202050.030.77053838-39
ACTCCGG228900.028.97646732-33
GAGCACA464250.028.8079079
CGGAGAA235550.028.22904436-37
TCGGAAG475200.027.8551643
AGAGCAC480500.027.8336568
ATCGGGG203400.027.6013942-43
TCCGGAG243900.027.26264834-35
CGGAAGA486650.027.2189834
CCGGAGA244750.027.13885734-35
CTCCGGA245250.027.09311532-33
ATCGGAA489350.026.8763852
GATCGGA498000.026.3883361
GTCACTC262100.025.35101528-29
ACGTCTG263250.025.1763314-15
AAGAGCA545900.024.4469817
ACACGTC276350.023.96569412-13
Produced by FastQC (version 0.11.5)