FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20612782
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1364860.662142548249916TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG167950.041.8548144-45
CGTATGC169450.041.7514546-47
TATGCCG171100.041.46142248-49
CTCGTAT170250.040.71745744-45
TCTCGTA180800.037.68403242-43
TACTCGA188900.037.2494336-37
TTACTCG194100.036.3127334-35
ACTCGAT191550.036.17621236-37
ATCTCGT191100.035.6032142-43
GTATGCC209900.033.89783546-47
ATGCCGT209350.033.8406948-49
CCGTCTT217000.032.67449652-53
GCCGTCT219600.032.1009950-51
CTCGATC219700.031.25994338-39
GAGCACA459550.031.118119
TCGATCT221300.030.80858638-39
AGAGCAC466350.030.623638
ATTACTC233550.030.53486334-35
ACATTAC236550.030.27793532-33
TCGGAAG470750.029.9954643
Produced by FastQC (version 0.11.5)