FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22140810
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1261370.5697036377621234TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG152500.041.19048344-45
CGTATGC155600.040.81324846-47
TATGCCG156150.040.62547348-49
CTCGTAT155550.039.94006744-45
TCTCGTA167500.036.33866542-43
GTCACGA181000.035.08364528-29
ATCTCGT179250.034.10236742-43
AGTCACG191350.033.43420428-29
TCACGAG198950.032.30049530-31
ACGAGAT199850.032.0956232-33
CGAGATT199100.031.9779732-33
GTATGCC201150.031.5593746-47
ATGCCGT201550.031.33300648-49
CCGTCTT206950.030.77162452-53
GCCGTCT206150.030.65820350-51
CACGAGA207750.030.65794830-31
CATCTCG211800.028.71565840-41
GAGCACA455250.028.5019999
ATCGGAA447250.028.3769662
AGAGCAC456200.028.3489468
Produced by FastQC (version 0.11.5)