FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22331805
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1392120.6233799730921885TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG169900.041.51514444-45
CGTATGC173050.041.03415746-47
TATGCCG175000.040.6462748-49
CTCGTAT174800.040.2291144-45
TATCTCG182150.038.33132640-41
TCTCGTA187750.037.31442642-43
ATCTCGT200650.035.16399442-43
ACCGCTC210250.033.88529232-33
GTATGCC216450.032.77355646-47
ATGCCGT218750.032.37587748-49
ATCGGAA440800.032.260422
GCCGTCT220850.031.8441550-51
CCGTCTT225350.031.54846452-53
TCGGAAG451600.031.4875763
CGCTCAT227400.031.43438134-35
GAGCACA461300.031.3703739
GATCGGA454550.031.220571
GTCACCG229250.031.02431928-29
AGAGCAC471550.030.6481938
CACCGCT233800.030.37034430-31
Produced by FastQC (version 0.11.5)