FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31560395
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT12702484.024816546180744TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT8337772.6418458957817226TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT1405260.4452605868842896TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTTT1269010.4020893908330362TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG644400.20417995402148803TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTGT343100.108712200845395TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3388350.083.6554951
ATCGGAA3398600.083.618692
TCGGAAG3426250.082.9951863
CGGAAGA3467950.082.015484
GAGCACA3464600.081.995319
AGAGCAC3469150.081.91248
AAGAGCA3577650.079.511877
GAAGAGC3643600.078.0994956
GGAAGAG3831100.074.407365
GAATCGG2814750.045.92632740-41
AATCGGG2791750.045.61480740-41
AGAATCG2948550.045.3134538-39
CACTCCG3130600.045.10588530-31
ACTCCGG3159250.044.9825932-33
ATCGGGG2620900.044.9699142-43
CGGAGAA3160500.044.880636-37
TCCGGAG3185700.044.60055534-35
CCGGAGA3164550.044.5969634-35
CACGTCT3246700.044.3299314-15
CGTCTGA3252400.044.2442816-17
Produced by FastQC (version 0.11.5)