FastQCFastQC Report
Fri 19 Apr 2019
H7J52BGXB_n01_AHm317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J52BGXB_n01_AHm317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21770071
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1699860.7808242793512249TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG211550.042.50133544-45
CGTATGC213100.042.29317546-47
TATGCCG215150.041.8699548-49
CTCGTAT213900.041.29052444-45
TACTCGA228650.039.3631736-37
ACTCGAT231350.038.34947236-37
TCTCGTA227000.038.30018642-43
TTACTCG237600.037.78060534-35
ATCTCGT240050.036.34665742-43
GTATGCC253450.035.54123746-47
ATGCCGT257850.034.9731248-49
GAGCACA522850.034.826529
AGAGCAC529750.034.4625558
ATCGGAA529050.034.1336252
CCGTCTT267500.033.80573352-53
TCGGAAG540250.033.460473
CTCGATC261500.033.33751738-39
GCCGTCT271150.033.2422650-51
GATCGGA545850.032.9794961
TCGATCT267550.032.4860338-39
Produced by FastQC (version 0.11.5)