FastQCFastQC Report
Sat 13 Apr 2019
H7J22BGXB_n01_ant10xantennae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7J22BGXB_n01_ant10xantennae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652211018
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA2013950.017.9992688
CTGCGGA1518050.017.9270941
CTCGAAA1722750.017.82731
AGGCGAA1032250.017.68295110
ATAACGC1793350.017.5864321
ACTGATG1590950.017.544691
GACTAAC1472050.017.5297161
CGTGAGC1392700.017.528411
TGACGGC1660600.017.449571
ACGAGGA1632800.017.3915651
ATAGACC1700700.017.367941
GATGAAA1769500.017.3503671
TTGAACG1631250.017.3404791
GACTGCG1818350.017.3186451
GGATTAC1950350.017.2543321
CGCTGGA1533650.017.23451
TTGGAAC1833950.017.2291761
TGACTAG1659300.017.2271231
CTCGAGG1647250.017.1752381
GTAACTG1977950.017.1240231
Produced by FastQC (version 0.11.5)