FastQCFastQC Report
Tue 4 Dec 2018
H7HWGAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28074332
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG876660.3122638857444587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG117600.012.70173270
AGAGCGG178950.09.30502870
GTAGGGG114750.08.5358770
GAGCGTG74750.08.14292570
TGTGCCG47950.07.66025370
CGGAAGC230450.07.42291670
CGGAAGG48300.07.097760770
CGCCGTA22250.06.9231098
AGCGCGG39450.06.56186370
GTGTGCC84800.06.39408370
TCGTGGG74150.06.08584670
AGTACGG32950.05.83915170
TCGTGTT409300.05.65794470
AGAGTGT502700.05.601663669
GTGCCGC37000.05.38738267
AGTGTGC102550.05.28720469
GAGTGTG298550.05.272758570
GGGAAAC90050.05.205512570
GGGAAAA184550.05.174769470
GTAGGGC54300.05.15385970
Produced by FastQC (version 0.11.5)