FastQCFastQC Report
Tue 13 Nov 2018
H7HWGAFXY_n02_pIXHALO_col_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HWGAFXY_n02_pIXHALO_col_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACACTACTTAGGCTTATAAGATCAGGATGAGGTTTAAGTTCTTATACTC17.6923076923076925No Hit
ATAGATCTCTAAAGCTAATGATAAGAGATAGAGAACAAGAAAAGTTAGAA17.6923076923076925No Hit
CAAACGGCCTAGGAGAATCCATGTCAGCGCATGCCATGACACGCCCATTC17.6923076923076925No Hit
TTCCAGAGATATTAGTTACTTGGCTAATAATCCAATTCTTCACCCACATC17.6923076923076925No Hit
AAAAAGCTGTAATGCTAGCAGATATGGCACAAATCACTGGTTTGATTGCT17.6923076923076925No Hit
AAATTTCATGATCTTAAAAAATGGAAAGTAAATTAGAGGGATGATAGCAA17.6923076923076925No Hit
TCACGCGGACTCGCGATCTTCAACAAGTATAGCCTTCGTTGATCCAAACC17.6923076923076925No Hit
CACGAAGTTAATTTTGAAACCCAACCCTTCCCGGTTTTACTCGGTTTAGG17.6923076923076925No Hit
CATGCATGATCTAGACTGAACTTCATTTTCATATCATTTTTAAAGAGCTC17.6923076923076925No Hit
GATACAAATCATCCTCCTTTTTGATCAGCTAGCGTCGTAATATCTTATAC17.6923076923076925No Hit
AACGGTGGCACCTAAACACGAGGGTTGCGCTCGTTGCGGGACTTAACCCA17.6923076923076925No Hit
CAATCATACACATGACATCAAGTCATATTCGACTGAAAAACACTAACCAA17.6923076923076925No Hit
AATTAGTAGTACCTAGAAAACTTCCTTAGACTGAGTAATGACTTAGGAAA17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)